United States - Oligo CVV Production

Description

View Changes UPDATE (09/11/2024): The Request for Quotes has been reopened and amended to clarify the quantity to be delivered. Quotes are now due 9/16/2024. See attached amended RFQ for details.
See Attached RFQ for Details and Terms
The Contractor shall provide the following:

Single-stranded DNA oligonucleotides:

Length is understood  to be a significant difference in the construction, processing, and pricing of oligos:
For single-stranded DNA oligonucleotides of up to 60 nucleotides (nt) in length:

The turnaround time (TAT) will be one (1) business day with an ordering cutoff of 10:00 a.m. EST (to be shipped same-day overnight for next-day delivery).
The expected minimum yield will be 25 nMole resuspended at 100 μM in nuclease-free water in a 96-well plate format, with ordering volumes of up to 8 plates per day.

For single-stranded DNA oligonucleotides of up to 80 nucleotides (nt) in length:

The turnaround time (TAT) will be one (1) business day with an ordering cutoff of 9:00 a.m. EST (to be shipped same-day overnight for next-day delivery).
The expected minimum yield will be 25 nMole resuspended in tubes at 100 μM in nuclease-free water.

DNA/gene fragments:

As with oligos, length is considered a significant factor in the manufacturing of gene fragments, and thus this task can be understood as having different expectations based on length.
Double-stranded DNA gene fragments of 300-1,500bp (base pairs) in length with an error rate of less than 1 bp in 5,000bp. The turnaround time (TAT) will be three (3) business days with an ordering cutoff of 11:00 a.m. EST (with the ordering date being day 0). The expected minimum yield will be 200ng delivered resuspended at 10 ng/µl in a minimum of 20 µl of nuclease-free water.
Double-stranded DNA gene fragments of 1,501-2,000bp (base pairs) in length with an error rate of less than 1 bp in 5,000bp. The turnaround time (TAT) will be eight (8) business days with an expected minimum yield of 1,000ng delivered lyophilized in tubes (with the ordering date being day 0).
Double-stranded DNA gene fragments of 2,001-2,250bp (base pairs) in length with an error rate of less than 1 bp in 5,000bp. The turnaround time (TAT) will be eight (8) business days with an expected minimum yield of 1,000ng delivered lyophilized in tubes (with the ordering date being day 0).

Opportunity closing date

16 September 2024

Value of contract

to be confirmed